Journal: The Journal of Experimental Medicine

Article Title: Interleukin 7 Transgenic Mice Develop Chronic Colitis with Decreased Interleukin 7 Protein Accumulation in the Colonic Mucosa

doi:

Figure Lengend Snippet: The phenotype of the infiltrating mucosal lymphocytes at the colitis lesion was analyzed by immunohistochemistry. Sections of colonic tissues from IL-7 transgenic mice were stained with anti-CD4 ( A ) and anti-CD8 ( B ) antibodies. Lymphoid infiltrates in the lamina propria at the colitis lesion were dominated by T cells bearing CD4 + . Both TCR-α/β T cells and γ/δ T cells infiltrated in the lamina propria of colonic mucosa (data not shown).

Article Snippet: mAbs used included anti-CD3, anti-CD4, anti-CD8α, anti-CD8β (Ly-3.2), anti–TCR-α/β, and anti–δ chain of TCR-γ/δ ( PharMingen ).

Techniques: Immunohistochemistry, Transgenic Assay, Staining

Journal: The Journal of Experimental Medicine

Article Title: Interleukin 7 Transgenic Mice Develop Chronic Colitis with Decreased Interleukin 7 Protein Accumulation in the Colonic Mucosa

doi:

Figure Lengend Snippet: The phenotype of the infiltrating mucosal lymphocytes at the colitis lesion was analyzed by immunohistochemistry. Sections of colonic tissues from IL-7 transgenic mice were stained with anti-CD4 ( A ) and anti-CD8 ( B ) antibodies. Lymphoid infiltrates in the lamina propria at the colitis lesion were dominated by T cells bearing CD4 + . Both TCR-α/β T cells and γ/δ T cells infiltrated in the lamina propria of colonic mucosa (data not shown).

Article Snippet: mAbs used included anti-CD3, anti-CD4, anti-CD8α, anti-CD8β (Ly-3.2), anti–TCR-α/β, and anti–δ chain of TCR-γ/δ ( PharMingen ).

Techniques: Immunohistochemistry, Transgenic Assay, Staining

Journal:

Article Title: CD4 + V?14 natural killer T cells are essential for acceptance of rat islet xenografts in mice

doi:

Figure Lengend Snippet: CD3+CD4+ cells in the liver. CD4/CD3 profiles of hepatic mononuclear cells and splenocytes of B6 mice (a and d). NK1.1 and IL-2Rβ expression on CD3intCD4+ cells (b and e) and CD3brightCD4+ (c and f).

Article Snippet: The following FITC-, biotin-, or phycoerythrin- conjugated mAb’s were purchased from PharMingen Co. (San Diego, California, USA); anti-CD3 (145-2C11), anti–IL-2 receptor β chain (TMβ1), anti-CD4 (GK1.5), anti-CD8α (53-6.7), anti-CD8β (53-5.8), anti-TCRβ (H57-597), and anti-NK1.1(PK136).

Techniques: Expressing

Journal:

Article Title: Characterization of extrathymic CD8?? T cells in the liver and intestine in TAP-1 deficient mice

doi: 10.1046/j.1365-2567.2003.01654.x

Figure Lengend Snippet: Presence of CD8+ T cells at extrathymic sites in TAP-1(−/−) mice. (a) Number of lymphocytes yielded by the spleen, liver, intestine (IEL = intraepithelial lymphocytes) and thymus (n = 4). (b) Phenotypic characterization of lymphocytes. Lymphocytes were obtained from both B6 and TAP-1(−/−) mice at the age of 12 weeks. Two-colour staining for CD4 and CD8α and for TCRαβ and TCRγδ was conducted. Numbers in the figure represent the percentages of fluorescence-positive cells in corresponding areas. The data shown here are representative of three experiments. *P < 0·05.

Article Snippet: The mAbs used here included anti-TCRαβ (H57-597), anti-TCRγδ (GL3), anti-CD3 (145–2C11), anti-IL-2Rβ (IL-2R β-chain; TM-β1), anti-NK1·1 (PK136), anti-CD4 (RM4-5), anti-CD8α (53–6·7) and CD8β (53–5·8) mAbs (PharMingen Co., San Diego, CA).

Techniques: Staining, Fluorescence

Journal:

Article Title: Characterization of extrathymic CD8?? T cells in the liver and intestine in TAP-1 deficient mice

doi: 10.1046/j.1365-2567.2003.01654.x

Figure Lengend Snippet: Further phenotypic characterization of lymphocytes in the livers and spleens of B6 and TAP-1(−/−) mice. (a) Two-colour staining for CD3 and IL-2Rβ and for CD3 and NK1·1. (b) Two-colour staining for CD4 and CD8α and for CD4 and CD8β. (c) Two-colour staining for TCRαβ and TCRγδ. In these experiments, young (12-week-old) and old (40-week-old) B6 and TAP-1(−/−) mice were used to show the expansion of CD8+ extrathymic T cells in the liver. Numbers in the figure represent the percentages of fluorescence-positive cells in corresponding areas. The data shown here are representative of three experiments.

Article Snippet: The mAbs used here included anti-TCRαβ (H57-597), anti-TCRγδ (GL3), anti-CD3 (145–2C11), anti-IL-2Rβ (IL-2R β-chain; TM-β1), anti-NK1·1 (PK136), anti-CD4 (RM4-5), anti-CD8α (53–6·7) and CD8β (53–5·8) mAbs (PharMingen Co., San Diego, CA).

Techniques: Staining, Fluorescence